摘  要:通过探讨了Abeta注射致痴呆模型建立的可行性及其行为学改变。方法：将适应性饲养后的SD品系雄性大鼠分组（模型组、对照组）进行造模，方法为给SD雄性大鼠双侧海马CA1区定向分别注射Abeta、生理盐水。待恢复10天后利用Morris 水迷宫和被动条件回避实验行为学检测手段来检测痴呆模型建立及其行为学改变。通过SPSS软件来分析所得数据间的差异性，并得出结论。结果：Morris水迷宫的结果显示对照组的找到平台的潜伏期明显的要比痴呆组的模型动物要短；被动条件回避实验结果则显示对照组进入黑箱的潜伏期要比痴呆组的长。结论：通过一系列的行为学检测实验证实Abeta注射致早期老年痴呆(AD)模型建立的可行性,并在短时间内模拟AD的神经元损伤和认知记忆能力障碍等行为学特征。

关键词：beta-淀粉样蛋白；阿尔茨海默病；Morris水迷宫；被动条件回避实验

Abstract:Discussing the feasibility of through the Abeta injection to establish AD animal model and discuss the animals’ model behavior change. Methods: Group male SD rats into the model group and the control group after two weeks adaptive raise. The model group is injecting Abeta to hippocampus CA1 area bilaterally. The control group is for inject the physiological saline. Restore 10 days before use the Morris water maze and passive reflex box to detect rats’ behavior change. Use the SPSS software to analyze the data and draw the conclusion. Results: the results of Morris water maze showed that the latency time before the rat in the control group find the platform is obviously shorter than the AD model animals which is induced by bilaterally injection Abeta to hippocampus. The Passive reflex box result shows that the latency time of the control group rat goes into the black box is longer than the AD model group. Conclusion: through a series of behavioral detection experiments we determine it is feasible that AD animal model can be established by injection Abeta, the AD model animal can also imitate the neuron damage caused by AD and raise the learning and memory disorder in a short period time.

Key Words：beta-amyloid peptide；Alzheimer's disease；Morris Water Maze；Passive Avoidance Task