摘要：采用酶学分析方法研究光照时间、水中钙、镁离子含量对龙虾肠内3种消化酶（蛋白酶、淀粉酶、脂肪酶）的活性的影响，并采用正交实验法对三个因素进行优选研究。实验结果表明：（1）在最优条件为：接受光照时间为自然光照、水中钙离子含量为25mg/L、镁离子含量为80mg/L，蛋白酶活力最大；（2）在最优条件为：接受光照时间为自然光照-4h、水中钙离子含量为35mg/L、镁离子含量为60mg/L，脂肪酶活力最大；（3）在最优条件为：接受光照时间为自然光照+4h、水中钙离子含量为35mg/L、镁离子含量为60mg/L，淀粉酶活力最大。同时，探究消化酶活力与龙虾生长之间的关系，结果表明：蛋白酶活力的大小与龙虾生长关系密切。

关键词：克氏源螯虾； 正交实验； 消化酶活力； 光照时间； 钙离子； 镁离子

Abstract: The effects of illumination time ,calcium ion and magnesium ion content in water on digestive enzyme activity of lobster were studied by the enzymatic analytical methed , and the use of orthogonal experimental methed to the three factors were optimized research .The results showed as follow : (1)under the optimal conditions : acceptance of the illumination time was the natural light,calcium concent in water was 25 mg / L, magnesium content in water was 80mg / L, the protease had the highest activities, (2)under the optimal conditions : acceptance of the illumination time was the natural light -4 h , calcium content in water was 35mg / L, magnesium content in water was 60mg / L,the lipase had the highest activities , (3) under the optimal conditions: acceptance illumination time was the natural light +4 h , calcium content in water was 35mg / L, magnesium content in water was 60mg / L, the amylase had the highest activities. At the same time , to expiore the relation between the digestive enzyme activity and the growth of the lobster, the results showed that : the protease activities were closely related with the growth of the lobster.

Keywords: Procambarus clarkia;  orthogonal experiment;  digestive enzyme activities;  illumination time;  calcium ion ;  magnesium ion