摘要:研究不同有机试剂萃取牛蒡乙醇提物的抗氧化性能，选择最优的进行分离纯化后研究各组份抗氧化性能,并对其有效成分结构做相应鉴定。牛蒡皮醇提物分别用石油醚、乙酸乙酯、正丁醇三种有机溶剂萃取，提取物采用ORAC法研究其抗氧化性，对最优组分采用高速逆流及高效液相色谱法进行分离纯化。四种牛蒡提取物中，乙酸乙酯萃取相的抗氧化性能最优；将乙酸乙酯提取物通过高速逆流进一步分离纯化，得出高速逆流收集相A的抗氧化性能优于其收集相B；对收集相A进行高效液相色谱分析，初步得出收集相A含有咖啡酸、绿原酸等多种成分，今后我们将对这些化合物做进一步的分析研究，并对其做结构鉴定。

关键词 牛蒡；乙醇提取物；抗氧化；分离纯化

Abstract:To study the different organic solvents extraction burdock ethanol-extraction content antioxidant properties, choose the best separation and purification study after the components oxidation resistance. And makes corresponding identification of the effective component structure. Burdock skin ethanol-extraction respectively with petroleum ether, ethyl acetate, n-buoh extraction, made by ORAC to find out the effect of remove oxidation resistance, separation and purification of the optimal components by HSCCC and HPLC. Burdock leather alcohol, petroleum ether, ethyl acetate, n-butanol extract, Burdock skin acetic ester extract oxidation resistance is the best. The ethyl acetate extract by HSCCC further purification, HSCCC drawn A antioxidant properties superior to its collection of B. To collect A HPLC analysis, preliminary concluded that collect drawn A caffeic acid, chlorogenic acid and other ingredients, in the future we will do further analysis and research of these compounds, and the structure identification.

Keywords  Burdock  ethanol-extraction  antioxidant  separation and purification